Overview

  • Product name
    Anti-LMO2 antibody [1A9-3D11]
    See all LMO2 primary antibodies
  • Description
    Mouse monoclonal [1A9-3D11] to LMO2
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to human LMO2.

  • Positive control
    • Human tonsil.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: Tissue culture supernatant
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab64139 was purified from ascites fluid using Protein A.
  • Clonality
    Monoclonal
  • Clone number
    1A9-3D11
  • Isotype
    IgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab64139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
    • Involvement in disease
      Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
    • Sequence similarities
      Contains 2 LIM zinc-binding domains.
    • Domain
      The second LIM zinc-binding domain interacts with KDM5A.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cysteine rich protein TTG 2 antibody
      • Cysteine rich protein TTG2 antibody
      • Cysteine-rich protein TTG-2 antibody
      • LIM domain only 2 (rhombotin like 1) antibody
      • LIM domain only 2 antibody
      • LIM domain only protein 2 antibody
      • LMO 2 antibody
      • LMO-2 antibody
      • lmo2 antibody
      • RBTN 2 antibody
      • RBTN L1 antibody
      • RBTN2 antibody
      • RBTN2_HUMAN antibody
      • RBTNL 1 antibody
      • RBTNL1 antibody
      • RHOM 2 antibody
      • RHOM2 antibody
      • Rhombotin 2 antibody
      • Rhombotin like 1 antibody
      • Rhombotin-2 antibody
      • Rhombotin2 antibody
      • T cell translocation gene 2 antibody
      • T cell translocation protein 2 antibody
      • T-cell translocation protein 2 antibody
      • TTG 2 antibody
      • TTG2 antibody
      see all

    Images

    • ab64139 staining LMO2 in human lymph node tissue section by Immunohistochemistry(Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, antigen retrieval in Target Retrieval Solution and blocking in 100% serum for 10 minutes at 25°C. The primary antibody was diluted, 1/700 and incubated with sample for 1 hour at 25°C. A polymer linked mouse polyclonal to mouse IgG1 was used undiluted, as secondary.

    References

    This product has been referenced in:
    • Funk KE  et al. Distinct Therapeutic Mechanisms of Tau Antibodies: Promoting Microglial Clearance Versus Blocking Neuronal Uptake. J Biol Chem 290:21652-62 (2015). Read more (PubMed: 26126828) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Specification
    Lymph node
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution,Dako
    Permeabilization
    No
    Blocking step
    Novolink Protein Block, Novocastra, Leica Microsystems Inc. as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Dr. Hajnalka Rajnai

    Verified customer

    Submitted Apr 01 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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