Anti-LMO2 antibody - ChIP Grade (ab72841)

Overview

  • Product nameAnti-LMO2 antibody - ChIP Grade
    See all LMO2 primary antibodies
  • Description
    Rabbit polyclonal to LMO2 - ChIP Grade
  • Tested applicationsSuitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal residues 1-100 of human LMO2. [Swiss-Prot# P25791]

  • Positive control
    • Ramos Whole Cell Extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 18 kDa).

Target

  • FunctionActs with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
  • Involvement in diseaseNote=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
  • Sequence similaritiesContains 2 LIM zinc-binding domains.
  • DomainThe second LIM zinc-binding domain interacts with KDM5A.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cysteine rich protein TTG 2 antibody
    • Cysteine rich protein TTG2 antibody
    • Cysteine-rich protein TTG-2 antibody
    • LIM domain only 2 (rhombotin like 1) antibody
    • LIM domain only 2 antibody
    • LIM domain only protein 2 antibody
    • LMO 2 antibody
    • LMO-2 antibody
    • lmo2 antibody
    • RBTN 2 antibody
    • RBTN L1 antibody
    • RBTN2 antibody
    • RBTN2_HUMAN antibody
    • RBTNL 1 antibody
    • RBTNL1 antibody
    • RHOM 2 antibody
    • RHOM2 antibody
    • Rhombotin 2 antibody
    • Rhombotin like 1 antibody
    • Rhombotin-2 antibody
    • Rhombotin2 antibody
    • T cell translocation gene 2 antibody
    • T cell translocation protein 2 antibody
    • T-cell translocation protein 2 antibody
    • TTG 2 antibody
    • TTG2 antibody
    see all

Anti-LMO2 antibody - ChIP Grade images

  • Anti-LMO2 antibody - ChIP Grade (ab72841) at 0.5 mg/ml + Ramos whole cell lysate

    Predicted band size : 18 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • ab72841 used undiluted in ChIP analysis, incubated for 16 hours at 4°C.
    Nuclear cell lysate prepared from mouse haematopoietic progenitor cell line HPC-7.
    Cross-linking (X-ChIP) performed by using 1% formaldehyde for 10 minutes.
    Detection step: Real time PCR.
    Negative control: A regultory element which is an open region of chromatin but is devoid of this transcription factor.

    See Abreview

References for Anti-LMO2 antibody - ChIP Grade (ab72841)

ab72841 has not yet been referenced specifically in any publications.

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The antibody ab26096 has not been tested in Chip application so I am sorry we are unable to confirm its use. This however does not means the antibody cannot be used it only means we do not have tested data. If you ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification haematopoietic progenitor cell line HPC-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control i) a competitors antibody known to work
ii) a regulatory element known to bind this transcription factor
Negative control a regultory element which is an open region of chromatin but is devoid of this transcription factor
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Submitted Feb 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"