Overview

  • Product name
    Anti-LMO2 antibody [EP3257]
    See all LMO2 primary antibodies
  • Description
    Rabbit monoclonal [EP3257] to LMO2
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Raji cell lysate and Fetal kidney lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    EP3257
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
  • Application notes
    Flow Cyt: 1/100 - 1/500.


    IP: 1/100 - 1/500.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.

    Is unsuitable for ICC or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
    • Involvement in disease
      Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
    • Sequence similarities
      Contains 2 LIM zinc-binding domains.
    • Domain
      The second LIM zinc-binding domain interacts with KDM5A.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cysteine rich protein TTG 2 antibody
      • Cysteine rich protein TTG2 antibody
      • Cysteine-rich protein TTG-2 antibody
      • LIM domain only 2 (rhombotin like 1) antibody
      • LIM domain only 2 antibody
      • LIM domain only protein 2 antibody
      • LMO 2 antibody
      • LMO-2 antibody
      • lmo2 antibody
      • RBTN 2 antibody
      • RBTN L1 antibody
      • RBTN2 antibody
      • RBTN2_HUMAN antibody
      • RBTNL 1 antibody
      • RBTNL1 antibody
      • RHOM 2 antibody
      • RHOM2 antibody
      • Rhombotin 2 antibody
      • Rhombotin like 1 antibody
      • Rhombotin-2 antibody
      • Rhombotin2 antibody
      • T cell translocation gene 2 antibody
      • T cell translocation protein 2 antibody
      • T-cell translocation protein 2 antibody
      • TTG 2 antibody
      • TTG2 antibody
      see all

    Anti-LMO2 antibody [EP3257] images

    • Overlay histogram showing Ramos cells stained with ab91652 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91652, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa

    References for Anti-LMO2 antibody [EP3257] (ab91652)

    This product has been referenced in:
    • Liu Y  et al. LMO2 Enhances Lamellipodia/Filopodia Formation in Basal-Type Breast Cancer Cells by Mediating ARP3-Profilin1 Interaction. Med Sci Monit 23:695-703 (2017). Read more (PubMed: 28170369) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab91652.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up