Overview

  • Product name
    Anti-LMO2 antibody [SP51] - N-terminal
    See all LMO2 primary antibodies
  • Description
    Rabbit monoclonal [SP51] to LMO2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Human LMO2 (N terminal). The exact sequence is proprietary.
    Database link: P25791

  • Positive control
    • Ramos cell lysate, Human tonsil tissue, Ramos cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purification notes
    Protien A/G purified
  • Clonality
    Monoclonal
  • Clone number
    SP51
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 18 kDa.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
  • Involvement in disease
    Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
  • Sequence similarities
    Contains 2 LIM zinc-binding domains.
  • Domain
    The second LIM zinc-binding domain interacts with KDM5A.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cysteine rich protein TTG 2 antibody
    • Cysteine rich protein TTG2 antibody
    • Cysteine-rich protein TTG-2 antibody
    • LIM domain only 2 (rhombotin like 1) antibody
    • LIM domain only 2 antibody
    • LIM domain only protein 2 antibody
    • LMO 2 antibody
    • LMO-2 antibody
    • lmo2 antibody
    • RBTN 2 antibody
    • RBTN L1 antibody
    • RBTN2 antibody
    • RBTN2_HUMAN antibody
    • RBTNL 1 antibody
    • RBTNL1 antibody
    • RHOM 2 antibody
    • RHOM2 antibody
    • Rhombotin 2 antibody
    • Rhombotin like 1 antibody
    • Rhombotin-2 antibody
    • Rhombotin2 antibody
    • T cell translocation gene 2 antibody
    • T cell translocation protein 2 antibody
    • T-cell translocation protein 2 antibody
    • TTG 2 antibody
    • TTG2 antibody
    see all

Images

  • Anti-LMO2 antibody [SP51] - N-terminal (ab183328) at 1/25 dilution + Ramos cell lysate

    Predicted band size: 18 kDa

  • Flow cytometric analysis of LMO2 in RAMOS cells using ab183328 (1/100)(green) compared to negative control of rabbit IgG (blue).

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LMO2 with ab183328 at 1/100.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LMO2 with ab183328 at 1/100.

References

This product has been referenced in:
  • Xie Y  et al. p53 expression is a strong marker of inferior survival in de novo diffuse large B-cell lymphoma and may have enhanced negative effect with MYC coexpression: a single institutional clinicopathologic study. Am J Clin Pathol 141:593-604 (2014). Read more (PubMed: 24619762) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
Yes - tween
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Methanol
Username

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Verified customer

Submitted Feb 08 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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