Anti-LMO7 antibody (ab224113)
Key features and details
- Rabbit polyclonal to LMO7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LMO7 antibody
See all LMO7 primary antibodies -
Description
Rabbit polyclonal to LMO7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human LMO7 aa 650-850.
Database link: Q8WWI1 -
Positive control
- WB: HeLa whole cell lysate (ab150035). ICC/IF: A431 cells. IHC-P: Human duodenum, skeletal muscle, testis and uterine cervix tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 154,158,185,191,193 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 154,158,185,191,193 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Tissue specificity
Widely expressed. Isoform 2 and isoform 4 are predominantly expressed in brain. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. - Information by UniProt
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Database links
- Entrez Gene: 4008 Human
- Omim: 604362 Human
- SwissProt: Q8WWI1 Human
- Unigene: 207631 Human
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Alternative names
- F box only protein 20 antibody
- F box protein Fbx20 antibody
- F-box only protein 20 antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for LMO7 (green) using ab224113 at 4 µg/ml in ICC/IF.
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Lane 1 : Anti-LMO7 antibody (ab224113) at 0.4 µg/ml (HeLa cell lysate)
Lane 2 : Anti-LMO7 antibody (ab224113) at 0.4 µg/ml (HEK-293 lysate)
All lanes :
Predicted band size: 154,158,185,191,193 kDaWestern blot analysis of HeLa cell lysate using ab224113 at 0.4 µg/ml.
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Paraffin-embedded human duodenum tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human uterine cervix tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224113 has not yet been referenced specifically in any publications.