The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects Approx. 70-75kDa band and approx. 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityWidely expressed. Isoform 2 and isoform 4 are predominantly expressed in brain.