Overview

  • Product nameAnti-LMX1A antibody
    See all LMX1A primary antibodies
  • Description
    Rabbit polyclonal to LMX1A
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse LMX1A.

    (Peptide available as ab31005.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue Lysate - normal tissue, 0 days old Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).Can be blocked with Mouse LMX1A peptide (ab31005).

Target

  • FunctionActs as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae.
  • Tissue specificityIsoform 1 is expressed in many tissues. Not found in heart, liver, spleen and testis. Relatively highly expressed in fetal brain. Isoform LMX1A-4B is expressed in testis.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HGNC:6653 antibody
    • LIM homeobox protein 1.1 antibody
    • LIM homeobox transcription factor 1 alpha antibody
    • LIM homeobox transcription factor 1-alpha antibody
    • LIM/homeobox protein 1.1 antibody
    • LIM/homeobox protein LMX1A antibody
    • LMX 1 antibody
    • LMX 1.1 antibody
    • LMX 1A antibody
    • LMX-1.1 antibody
    • LMX1 antibody
    • LMX1.1 antibody
    • LMX1A antibody
    • LMX1A_HUMAN antibody
    • MGC87616 antibody
    see all

Anti-LMX1A antibody images

  • All lanes : Anti-LMX1A antibody (ab31006) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-LMX1A antibody (ab31006)

This product has been referenced in:
  • Esfandiari F  et al. Glycogen synthase kinase-3 inhibition promotes proliferation and neuronal differentiation of human-induced pluripotent stem cell-derived neural progenitors. Stem Cells Dev 21:3233-43 (2012). WB ; Human . Read more (PubMed: 22642687) »
  • Rössler R  et al. Differentiation of non-mesencephalic neural stem cells towards dopaminergic neurons. Neuroscience 170:417-28 (2010). WB ; Mouse . Read more (PubMed: 20643196) »

See all 2 Publications for this product

Product Wall

Thank you for sending your protocol. I am sorry to see that this antibody and the LMX1a antibody ab31006 are both giving negative IHC results.

I think your protocol is good and I would expect to see a positive result for ab66941, though we h...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (human nueroblastoma cell)
Loading amount 50 µg
Specification human nueroblastoma cell
Treatment MG132 40uM 16hrs
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse midbrain)
Specification Mouse midbrain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Miss. Joanna Brent

Verified customer

Submitted Apr 07 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult brain and embryo torso T/S)
Specification adult brain and embryo torso T/S
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 18 2010

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Embryonic brain)
Specification Embryonic brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Foetal midbrain E15+2 DIC)
Specification Foetal midbrain E15+2 DIC
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Miss. Miriam Gubernator

Verified customer

Submitted Dec 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"