Anti-LMX1b antibody (ab66941)

Overview

  • Product name
  • Description
    Rabbit polyclonal to LMX1b
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Zebrafish
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide: LAPPQQQIVA MEQSPYGSSD PFQQGLTPPQ MPGNDSIFHD IDSDTSLTSL, corresponding to amino acids 289-338 of LMX1b

Properties

Applications

Our Abpromise guarantee covers the use of ab66941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
  • Tissue specificity
    Expressed in most tissues. Highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets.
  • Involvement in disease
    Defects in LMX1B are the cause of nail-patella syndrome (NPS) [MIM:161200]; also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM homeo box transcription factor 1 beta antibody
    • LIM homeobox transcription factor 1 beta antibody
    • LIM homeobox transcription factor 1-beta antibody
    • LIM-homeobox protein 1.2 antibody
    • LIM/homeobox protein 1.2 antibody
    • LIM/homeobox protein LMX1B antibody
    • LMX 1.2 antibody
    • LMX-1.2 antibody
    • LMX1.2 antibody
    • LMX1B antibody
    • LMX1B_HUMAN antibody
    • MGC138325 antibody
    • MGC142051 antibody
    • NPS 1 antibody
    • NPS1 antibody
    see all

Images



  • Predicted band size : 41 kDa

References

ab66941 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your phone call.

This antibody was validated on frozen sections by a customer:

http://www.abcam.com/index.html?datasheet=66941&tab=abreviews&intabreviewid=19533

We would recommend a light fixation, su...

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Your details has been forwarded to me by my colleague.

I hope the new vial will work for you; in case of any problem please do not hesitate to contact me.

Best of luck with you research!

Thank you for sending your protocol. I am sorry to see that this antibody and the LMX1a antibody ab31006 are both giving negative IHC results.

I think your protocol is good and I would expect to see a positive result for ab66941, though we h...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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We have 3 more antibodies in catalogue ab70174, ab82835 and ab129306. Which one you would be interested in getting?

Thank you for sending the questionnaire.

It is difficult to guess, the identity of band at 30kDa which is consistent in all lysates. The only lysate which shows correct band size is LMX1B trans-fected Hek 293 cells. In regards to other lysate...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (heart, fin)
Specification
heart, fin
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Tween 20 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jul 05 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic brain)
Specification
Embryonic brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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