Recombinant
RabMAb

Anti-LNK antibody [EPR7342] (ab154848)

Overview

  • Product name
    Anti-LNK antibody [EPR7342]
    See all LNK primary antibodies
  • Description
    Rabbit monoclonal [EPR7342] to LNK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human LNK (Uniprot: Q9UQQ2)

  • Positive control
    • Ramos, MOLT4 and Raji cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
    • Tissue specificity
      Preferentially expressed by lymphoid cell lines.
    • Involvement in disease
      Genetic variations in SH2B3 are associated with susceptibility to celiac disease type 13 (CELIAC13)[MIM:612011]; also known as susceptibility to gluten-sensitive enteropathy type 13. Celiac disease is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
      Genetic variations in SH2B3 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]; also known as diabetes mellitus type 1. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
    • Sequence similarities
      Belongs to the SH2B adapter family.
      Contains 1 PH domain.
      Contains 1 SH2 domain.
    • Post-translational
      modifications
      Tyrosine phosphorylated by LCK.
    • Information by UniProt
    • Database links
    • Alternative names
      • IDDM20 antibody
      • Lymphocyte adapter protein antibody
      • Lymphocyte specific adapter protein antibody
      • Lymphocyte specific adapter protein Lnk antibody
      • Lymphocyte-specific adapter protein Lnk antibody
      • SH2B adapter protein 3 antibody
      • SH2B adaptor protein 3 antibody
      • Sh2b3 antibody
      • SH2B3_HUMAN antibody
      • Signal transduction protein Lnk antibody
      see all

    Images

    • All lanes : Anti-LNK antibody [EPR7342] (ab154848) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 63 kDa

    References

    ab154848 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (iPSC derived blood cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100 µg
    Specification
    iPSC derived blood cell
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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