Overview

  • Product nameAnti-Lnx1 antibodySee all Lnx1 primary antibodies ...
  • Description
    Mouse polyclonal to Lnx1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Cow
  • Immunogen

    Recombinant fusion protein: MNQPDLADDPDPSPEPLCIV, corresponding to N terminal amino acids 1-20 of Mouse Lnx1.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab43692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 80 kDa.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceLnx1 is a membrane-bound protein that is involved in signal transduction and protein interactions. Lnx1 is an E3 Ubiquitin ligase protein that mediates ubiquitination and subsequent proteasomal degradation of proteins containing phosphotyrosine binding (PTB) domains. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Lnx1 mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • E3 ubiquitin protein ligase LNX antibody
      • Ligand of Numb protein X 1 antibody
      • Ligand of numb protein X 1 E3 ubiquitin protein ligase antibody
      • LNX antibody
      • LNX1 antibody
      • MPDZ antibody
      • Multi PDZ domain containing protein E3 ubiquitin protein ligase LNX antibody
      • Numb binding protein 1 antibody
      • PDZ domain-containing ring finger protein 2 antibody
      • PDZRN2 antibody
      see all

    Anti-Lnx1 antibody images

    • All lanes : Anti-Lnx1 antibody (ab43692) at 1/1000 dilution

      Lane 1 : (left) 20µg of a total protein extract from E coli with ~50ng to 500ng of a tagged fusion protein of an irrelevant antigen.
      Lane 2 : (Right) 20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (Tagged fusion protein).

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

      Predicted band size : 80 kDa

    References for Anti-Lnx1 antibody (ab43692)

    ab43692 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"