Overview

  • Product nameAnti-LOC285498 antibody
    See all LOC285498 primary antibodies
  • Description
    Rabbit polyclonal to LOC285498
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 72 - 121 (LCKKYSRETS QILEFQEKHR KRLLAFYREK ISRLEESLRK SVLQIEQLQS) of Human LOC285498 (NP_919420)

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionKey regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May act as a SUMO E3 ligase that mediates sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Cellular localizationNucleus. Chromosome. Associates to the synaptonemal complex. Localizes to a minority of double-strand breaks (DSBs) sites. Marks crossover sites during midpachynema.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ38841 antibody
    • Hypothetical protein LOC285498 antibody
    • OTTHUMP00000147525 antibody
    • Probable E3 SUMO-protein ligase RNF212 antibody
    • RING finger protein 212 antibody
    • RN212_HUMAN antibody
    • RNF 212 antibody
    • RNF212 antibody
    • ZHP3 antibody
    • ZHP3, C. elegans, homolog of antibody
    • ZIP3-related protein antibody
    see all

Anti-LOC285498 antibody images

  • Anti-LOC285498 antibody (ab83874) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,55 kDa. We are unsure as to the identity of these extra bands.

References for Anti-LOC285498 antibody (ab83874)

ab83874 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"