Overview

  • Product name
  • Description
    Rabbit polyclonal to LOC51035
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 106-324 of Human LOC51035.

  • Positive control
    • 293T, A431, HeLa, NIH-3T3, JC and BCL-1 whole cell lysates; HeLa cells; Human colon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist.
  • Cellular localization
    Cytoplasm
  • Database links
  • Alternative names
    • 2B28 antibody
    • 4930455J02Rik antibody
    • D19Ertd721e antibody
    • MGC124706 antibody
    • MGC6696 antibody
    • mY33K antibody
    • Protein 2B28 antibody
    • RGD1309471 antibody
    • SAKS1 antibody
    • SAPK substrate protein 1 antibody
    • T25529 antibody
    • UBA/UBX 33.3 kDa protein antibody
    • UBX domain containing protein 1 antibody
    • UBX domain protein 1 antibody
    • UBXD10 antibody
    • UBXN1 antibody
    see all

Images

  • All lanes : Anti-LOC51035 antibody (ab154265) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate

    Lane 2 : A431 whole cell lysate

    Lane 3 : HeLa whole cell lysate


    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS PAGE
  • All lanes : Anti-LOC51035 antibody (ab154265) at 1/3000 dilution

    Lane 1 : NIH-3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling LOC51035 with ab154265 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling LOC51035 with ab154265 at 1/500 dilution.

References

ab154265 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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