Recombinant
RabMAb

Anti-LOC51035 antibody [EPR9136] (ab151723)

Overview

  • Product name
    Anti-LOC51035 antibody [EPR9136]
    See all LOC51035 primary antibodies
  • Description
    Rabbit monoclonal [EPR9136] to LOC51035
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human LOC51035 (UniProt: Q04323).

  • Positive control
    • HeLa, HepG2, 293T and Ramos cell lysates; Human prostate and testis tissues; 293T cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist.
    • Cellular localization
      Cytoplasm
    • Database links
    • Alternative names
      • 2B28 antibody
      • 4930455J02Rik antibody
      • D19Ertd721e antibody
      • MGC124706 antibody
      • MGC6696 antibody
      • mY33K antibody
      • Protein 2B28 antibody
      • RGD1309471 antibody
      • SAKS1 antibody
      • SAPK substrate protein 1 antibody
      • T25529 antibody
      • UBA/UBX 33.3 kDa protein antibody
      • UBX domain containing protein 1 antibody
      • UBX domain protein 1 antibody
      • UBXD10 antibody
      • UBXN1 antibody
      see all

    Images

    • All lanes : Anti-LOC51035 antibody [EPR9136] (ab151723) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 33 kDa

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling LOC51035 with ab151723 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling LOC51035 with ab151723 at 1/50 dilution.
    • Flow cytometry analysis of permeabilized 293T cells labeling LOC51035 with ab151723 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
    • Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab151723 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151723 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151723 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151723 showing +ve staining.

    References

    ab151723 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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