Overview

  • Product nameAnti-LOK antibody
    See all LOK primary antibodies
  • Description
    Rabbit polyclonal to LOK
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LOK aa 692-721 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O94804

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human hepatocarcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab37727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 112 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionCan act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
  • Tissue specificityExpressed predominantly in lymphoid organs.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOK antibody
    • Lymphocyte oriented kinase antibody
    • Lymphocyte-oriented kinase antibody
    • PRO2729 antibody
    • Serine threonine protein kinase 10 antibody
    • Serine/threonine-protein kinase 10 antibody
    • STK10 antibody
    • STK10_HUMAN antibody
    see all

Anti-LOK antibody images

  • Anti-LOK antibody (ab37727) + Jurkat cell lysate

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling LOK with ab37727. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

References for Anti-LOK antibody (ab37727)

ab37727 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lymphoblast cells +/+ and +/- Stk10)
Loading amount 25 µg
Specification lymphoblast cells +/+ and +/- Stk10
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Feb 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"