All lanes : Anti-LOK antibody (ab70484) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique
Predicted band size : 112 kDa Observed band size : 112 kDa
Immunoprecipitation - LOK antibody (ab70484)
Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
Lane 1: input of Human LOK at 50µg
Lane 2: Other anti-human LOK antibody
Lane 3: Other anti-human LOK antibody plus blocking peptide
Lane 4: ab70484
Lane 5: ab70484 plus blocking peptide
Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
Chemiluminescence with exposure times of 1 to 2 minutes.