Overview

  • Product nameAnti-LOK antibody
    See all LOK primary antibodies
  • Description
    Rabbit polyclonal to LOK
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 900-968 of Human LOK

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab70484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/15000. Predicted molecular weight: 112 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
    • Tissue specificityExpressed predominantly in lymphoid organs.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • LOK antibody
      • Lymphocyte oriented kinase antibody
      • Lymphocyte-oriented kinase antibody
      • PRO2729 antibody
      • Serine threonine protein kinase 10 antibody
      • Serine/threonine-protein kinase 10 antibody
      • STK10 antibody
      • STK10_HUMAN antibody
      see all

    Anti-LOK antibody images

    • All lanes : Anti-LOK antibody (ab70484) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 112 kDa
    • Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
      Lane 1: input of Human LOK at 50µg
      Lane 2: Other anti-human LOK antibody
      Lane 3: Other anti-human LOK antibody plus blocking peptide
      Lane 4: ab70484
      Lane 5: ab70484 plus blocking peptide
      Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
      Chemiluminescence with exposure times of 1 to 2 minutes.

    References for Anti-LOK antibody (ab70484)

    ab70484 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70484.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"