Overview

  • Product name
  • Description
    Rabbit polyclonal to LOK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 900-968 of Human LOK

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 1-4µg/mg of lysate.
    WB: 1/2000 - 1/15000. Predicted molecular weight: 112 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
    • Tissue specificity
      Expressed predominantly in lymphoid organs.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • LOK antibody
      • Lymphocyte oriented kinase antibody
      • Lymphocyte-oriented kinase antibody
      • PRO2729 antibody
      • Serine threonine protein kinase 10 antibody
      • Serine/threonine-protein kinase 10 antibody
      • STK10 antibody
      • STK10_HUMAN antibody
      see all

    Images

    • All lanes : Anti-LOK antibody (ab70484) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 5 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 112 kDa
    • Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
      Lane 1: input of Human LOK at 50µg
      Lane 2: Other anti-human LOK antibody
      Lane 3: Other anti-human LOK antibody plus blocking peptide
      Lane 4: ab70484
      Lane 5: ab70484 plus blocking peptide
      Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
      Chemiluminescence with exposure times of 1 to 2 minutes.

    References

    ab70484 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70484.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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