Overview

  • Product nameAnti-LONRF1 antibody
    See all LONRF1 primary antibodies
  • Description
    Rabbit polyclonal to LONRF1
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: MSSPAVARTS PGGSREMAPA PQGRGRFWEV GGGSGHRLER AAAESERWEL, corresponding to N terminal amino acids 1-50 of Human LONRF1

  • Positive control
    • HepG2 cell lysate and Human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • RelevanceContains 1 Lon domain. Contains 1 RING-type zinc finger.
  • Database links
  • Alternative names
    • LON peptidase N-terminal domain and RING finger protein 1 antibody
    • RING finger protein 191 antibody
    • RNF191 antibody

Anti-LONRF1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling LONRF1 with ab60099 at 4-8µg/ml. Epithelial cells of the renal tubule show positive staining. Magnification: 400X.

  • Anti-LONRF1 antibody (ab60099) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-LONRF1 antibody (ab60099)

ab60099 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"