Anti-Loquacious antibody (ab24237)

Overview

  • Product name
    Anti-Loquacious antibody
  • Description
    Rabbit polyclonal to Loquacious
  • Specificity
    ab24237 specifically recognises recombinant Loquacious fragment protein. However, ab24237 does not appear to detect endogenous Loquacious in Drosophila embryo lysate, which may be due to low expression in this lysate.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Recombinant fragment derived from within residues 300 to the C-terminus of Fruit fly (Drosophila melanogaster) Loquacious.

  • Positive control
    • This antibody gave a positive signal in the following recombinant protein: Loquacious recombinant (immunizing) fragment protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.

Target

  • Relevance
    Loquacious is a Drosophila double-stranded RNA binding protein that is required for efficient microRNA-mediated gene silencing by Dicer-1. microRNAs (miRNAs) are single-stranded, 21- to 23-nucleotide cellular RNAs that control the expression of cognate target genes.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • CG6866 antibody
      • loqs antibody
      • R3D1 antibody
      • R3D1 L antibody
      • R3D1 S antibody
      • TRBP antibody
      see all

    Anti-Loquacious antibody images

    • All lanes : Anti-Loquacious antibody (ab24237) at 1 µg/ml

      Lane 1 : Loquacious recombinant fragment protein
      Lane 2 : Drosophila embryo lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Predicted band size : 45 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References for Anti-Loquacious antibody (ab24237)

    ab24237 has not yet been referenced specifically in any publications.

    Product Wall


    I found the information about Loquacious on SwissProt : http://www.uniprot.org/uniprot/?query=Loquacious+AND+organism%3A%22Drosophila+melanogaster+%5B7227%5D%22&sort=score

    Please note that the links to each isoform are also on the pro...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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