• Product nameAnti-Loricrin antibody
    See all Loricrin primary antibodies
  • Description
    Rabbit polyclonal to Loricrin
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Loricrin aa 450 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab95418, ab95418)

  • Positive control
    • This antibody gave a positive signal in T47D (Human breast duct epithelial carcinoma) whole cell lysate. IHC-P: FFPE human normal skin tissue sections.



Our Abpromise guarantee covers the use of ab85679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26,37 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionMajor keratinocyte cell envelope protein.
  • Involvement in diseaseDefects in LOR are a cause of progressive symmetric erythrokeratodermia (PSEK) [MIM:133200]. Erythrokeratodermas are a group of disorders characterized by widespread erythematous plaques, either stationary or migratory, associated with features that include palmoplantar keratoderma. PSEK is characterized by erythematous and hyperkeratotic plaques.
    Defects in LOR are the cause of Vohwinkel syndrome with ichthyosis (VSI) [MIM:604117]; also known as loricrin keratoderma (LK) or mutilating keratoderma with ichthyosis. VSI is an ichthyotic variant of Vohwinkel syndrome (VS) characterized by progressive symmetric erythrokeratoderma or congenital ichthyosiform erythroderma born as a collodion baby. Common clinical features include hyperkeratosis of the palms and soles with digital constriction.
  • Post-translational
    Substrate of transglutaminases. Some glutamines and lysines are cross-linked to other loricrin molecules and to SPRRs proteins.
    Contains inter- or intramolecular disulfide-bonds.
  • Cellular localizationCytoplasm. Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOR antibody
    • LOR protein antibody
    • LORI_HUMAN antibody
    • Loricrin antibody
    • LRN antibody
    • MGC111513 antibody
    • OTTHUMP00000015823 antibody
    see all

Anti-Loricrin antibody images

  • IHC image of Loricrin staining in human normal skin formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85679, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ICC/IF image of ab85679 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85679, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-Loricrin antibody (ab85679) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Mouse skin tissue lysate - total protein (ab4025)

    Lysates/proteins at 20 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 26,37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab85679 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Loricrin has a predicted molecular weight of 26 kDa in human and 38 kDa in mouse (Swissprot data).

References for Anti-Loricrin antibody (ab85679)

This product has been referenced in:
  • Mallery SR  et al. Topical application of a mucoadhesive freeze-dried black raspberry gel induces clinical and histologic regression and reduces loss of heterozygosity events in premalignant oral intraepithelial lesions: results from a multicentered, placebo-controlled clinical trial. Clin Cancer Res 20:1910-24 (2014). WB ; Human . Read more (PubMed: 24486592) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EnVision™ FLEX Target Retrieval Solution, High pH (50x). Tris/EDTA buffer, pH 9 from Dako
Permeabilization No
Specification Skin
Blocking step EnVision™ FLEX Peroxidase-Blocking Reagent as blocking agent as blocking agent for 5 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jan 17 2017

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde

Ahmar Aziz

Verified customer

Submitted Apr 05 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100 mM citrate buffer, pH 6
Sample Mouse Tissue sections (cornifying epithelia)
Specification cornifying epithelia
Permeabilization No
Fixative Formaldehyde

Mr. Albert Ricken

Verified customer

Submitted Mar 25 2015

Merci pour votre réponse.

Les deux anti-loricrine ab24722 et ab85679ont étéretestés par notre laboratoire en IHC et les deux anticorps ont été validés comme fonctionnels et spécifiques. L...

Read More

Merci pour votre réponse.

Je viens de relancer mes collègues du laboratoire qui ont retesté cet anticorps. Je vous contacterai de nouveau dès que j'aurai reçu les résultats et les conclusions.

Read More

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (dorsal skin)
Specification dorsal skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Hsu Fu-Chih

Verified customer

Submitted Jun 11 2012

Merci pour ce complément d'informations.

Pourriez-vous m'envoyer quelques détails supplémentaires de votre protocole en remplissant le questionnaire ci-joint?


Expiration date: 11/05/2012

I am very pleased to hear you would like to accept our offer and test ab85679 in [Mouse]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the...

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Merci de nous avoir contactés.

Mes collaborateurs et moi-même travaillons toujours sur la question des anti-loricrin ab85679 et ab24722.

Pourriez-vous nous envoyer une image du blot obtenu avec ab85679 ?