Potent, competitive HMG-CoA reductase inhibitor (Ki = 0.6 nM). Blood-brain barrier permeable. Inhibits in vivo and in vitro cholesterol biosynthesis. Also reduces α-synuclein levels in vitro and in vivo.
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in DMSO to 100 mM and in ethanol to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab87359 staining cyclin A in DU145 cells treated with lovastatin (ab120614), by ICC/IF. Decrease in cyclin A expression correlates with increased concentration of lovastatin, as described in literature. The cells were incubated at 37°C for 24h in media containing different concentrations of ab120614 (lovastatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab87359 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Koob AO et al. Lovastatin ameliorates alpha-synuclein accumulation and oxidation in transgenic mouse models of alpha-synucleinopathies. Exp Neurol221:267-74 (2010).
Read more (PubMed: 19944097) »
Chen GZ et al. Purification and characterization of 3-hydroxymethylglutaryl-coenzyme A reductase of Schistosoma mansoni: regulation of parasite enzyme activity differs from mammalian host. Exp Parasitol73:82-92 (1991).
Read more (PubMed: 1905241) »
Singer II et al. Lovastatin, an inhibitor of cholesterol synthesis, induces hydroxymethylglutaryl-coenzyme A reductase directly on membranes of expanded smooth endoplasmic reticulum in rat hepatocytes. Proc Natl Acad Sci U S A85:5264-8 (1988).
Read more (PubMed: 3293052) »