Overview

  • Product nameAnti-LOX 1 antibody
    See all LOX 1 primary antibodies
  • Description
    Rabbit polyclonal to LOX 1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LOX 1.

    (Peptide available as ab69660.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Brain (Human) Spinal Cord (Human) IF/ICC: JEG-3 cell line.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionReceptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
  • Tissue specificityExpressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
  • Involvement in diseaseNote=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
    Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe cytoplasmic region is required for subcellular sorting on the cell surface.
    The C-type lectin domain mediates the recognition and binding of oxLDL.
  • Post-translational
    modifications
    The intrachain disulfide-bonds prevent N-glycosylation at some sites.
    N-glycosylated.
  • Cellular localizationCell membrane. Secreted. A secreted form also exists.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-type lectin domain family 8 member A antibody
    • CLEC8A antibody
    • hLOX 1 antibody
    • hLOX-1 antibody
    • Lectin like oxidized LDL receptor 1 antibody
    • Lectin like oxLDL receptor 1 antibody
    • Lectin type oxidized LDL receptor 1 antibody
    • Lectin-like oxidized LDL receptor 1 antibody
    • Lectin-like oxLDL receptor 1 antibody
    • Lectin-type oxidized LDL receptor 1 antibody
    • low density lipoprotein oxidized, receptor 1 antibody
    • LOX-1 antibody
    • LOXIN antibody
    • Olr1 antibody
    • OLR1_HUMAN antibody
    • Ox LDL receptor 1 antibody
    • Ox-LDL receptor 1 antibody
    • Oxidised low density lipoprotein (lectin like) receptor 1 antibody
    • Oxidized low density lipoprotein receptor 1 antibody
    • Oxidized low density lipoprotein receptor 1 soluble form antibody
    • Oxidized low-density lipoprotein receptor 1 antibody
    • SCARE1 antibody
    • Scavenger receptor class E, member 1 antibody
    • SLOX1 antibody
    • soluble form antibody
    • SR-EI antibody
    see all

Anti-LOX 1 antibody images

  • ICC/IF image of ab65864 stained JEG-3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65864, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-LOX 1 antibody (ab65864) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466) at 10 µg
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188) at 5 µg
    Lane 3 : Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 42 kDa (possible isoform,glycosylated form),51 kDa (possible isoform,glycosylated form).

    Exposure time : 1 minute
    LOX1 contains a number of potentially glycosylated isoforms (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References for Anti-LOX 1 antibody (ab65864)

This product has been referenced in:
  • Hilgendorff A  et al. Lung matrix and vascular remodeling in mechanically ventilated elastin haploinsufficient newborn mice. Am J Physiol Lung Cell Mol Physiol 308:L464-78 (2015). WB ; Mouse . Read more (PubMed: 25539853) »

See 1 Publication for this product

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Unfortunately, I have to confirm that the antibodies we have in our catalog are unlikely to definitively not react with pig.

The whole protein shows ca 70% homology between human and pig. Therefore we would ...

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