Overview

  • Product nameAnti-LOX antibody
    See all LOX primary antibodies
  • Description
    Rabbit polyclonal to LOX
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Dog
  • Immunogen

    Synthetic peptide derived from within residues 350 to the C-terminus of Human LOX.

    (Peptide available as ab28612.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: MDA-MB-361, MDA-MB-231, MCF-7, and MEF1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab31238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human LOX peptide (ab28612).

Abcam recommends using Milk as the blocking agent.

Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100. PubMed: 22685574
IHC-FoFr 1/300.

Target

  • FunctionResponsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
  • Tissue specificityHeart, placenta, skeletal muscle, kidney, lung and pancreas.
  • Involvement in diseaseDefects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
  • Sequence similaritiesBelongs to the lysyl oxidase family.
  • Post-translational
    modifications
    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • lox antibody
    • LYOX antibody
    • LYOX_HUMAN antibody
    • Lysyl oxidase antibody
    • MGC105112 antibody
    • Protein lysine 6 oxidase antibody
    • Protein-lysine 6-oxidase antibody
    see all

Anti-LOX antibody images

  • ab31238 staining LOX in human saphenous vein tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween 20 and blocked with 15% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 10% serum in TBS + 0.05% Tween) for 14 hours at 4°C. An Alexa Fluor® 546-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Lanes 1 - 4 : Anti-LOX antibody (ab31238) at 1 µg/ml (Milk blocking)
    Lanes 5 - 8 : Anti-LOX antibody (ab31238) at 1 µg/ml (BSA blocking)

    Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 8 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk (lanes 1-4) or 2% Bovine Serum Albumin (lanes 5-8) before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • Anti-LOX antibody (ab31238) at 1 µg/ml + MDA-MB-361 whole cell lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab31238 staining LOX in adult rat cardiac fibroblast cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100. The sample was incubated with the primary antibody (1µg/cells in PBS + BSA) for 45 minutes at 4°C. A FITC-conjugated goat anti-rabbit IgG (1/160) was used as the secondary antibody.
    Gating Strategy: 10000 cells.

    See Abreview

  • ab31238 staining LOX in mouse 15.5dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS in TBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-LOX antibody (ab31238) at 1 µg/ml

    Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Additional bands at : 15 kDa,36 kDa (possible mature (processed) protein),47 kDa (possible immature (unprocessed)),52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31238 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemical analysis of murine embryonic spinal tissue, staining LOX with ab31238.

    Tissue was permeabilized with 0.025% Triton-X and blocked for nonspecific binding using blocking solution. Sections were incubated overnight with primary antibody (1/100) and staining was detected using DAB.

References for Anti-LOX antibody (ab31238)

This product has been referenced in:
  • Chang HM  et al. Activin A-induced increase in LOX activity in human granulosa-lutein cells is mediated by CTGF. Reproduction 152:293-301 (2016). Human . Read more (PubMed: 27530347) »
  • Miana M  et al. The lysyl oxidase inhibitor ß-aminopropionitrile reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats. Dis Model Mech 8:543-51 (2015). WB ; Rat . Read more (PubMed: 26035864) »

See all 15 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (Secreted media from LOX-overexpressing cells)
Total protein in input 200 µg
Treatment Control and treated with protease X
Immuno-precipitation step Protein A/G
Specification Secreted media from LOX-overexpressing cells
Username

Dr. Fernando Rodrguez-Pascual

Verified customer

Submitted Sep 27 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Renal tubular primary cell culture)
Permeabilization Yes - Triton
Specification Renal tubular primary cell culture
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ms. Barbara Torsello

Verified customer

Submitted Mar 14 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Heart)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinas K - Ready to use (Dako - S3020)
Permeabilization No
Specification Heart
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Adipose tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate ph6
Permeabilization Yes - Tween 1%
Specification Adipose tissue
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 20 2015

Application Western blot
Sample Rat Tissue lysate - whole (white adipose tissue)
Gel Running Conditions Reduced Denaturing (7%)
Loading amount 20 µg
Treatment control and high fat diet-fed rats
Specification white adipose tissue
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1000µg/mL · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 16 2015

Application Western blot
Sample Human Cell lysate - other (VSMC supernatant)
Gel Running Conditions Non-reduced Denaturing (7%)
Loading amount 20 µg
Specification VSMC supernatant
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1000µg/mL · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 28 2015

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 0,3% Triton X-100
Sample Rat Cell (adult Cardiac Fibroblast)
Specification adult Cardiac Fibroblast
Gating Strategy 10000 cells
Preparation Cell harvesting/tissue preparation method: Trip-EDTA
Sample buffer: PBS-BSA
Username

Abcam user community

Verified customer

Submitted Jul 18 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 15% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Sample Human Tissue sections (saphenous vein)
Specification saphenous vein
Permeabilization Yes - Tween20
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 14 2014

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