Overview

  • Product nameLOX1 (OLR1) Mouse ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse LOX1 (OLR1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse LOX1 in cell culture supernatants, serum and plasma (heparin and EDTA)

    A LOX1 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for LOX1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse LOX1 amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionReceptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
  • Tissue specificityExpressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
  • Involvement in diseaseNote=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
    Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe cytoplasmic region is required for subcellular sorting on the cell surface.
    The C-type lectin domain mediates the recognition and binding of oxLDL.
  • Post-translational
    modifications
    The intrachain disulfide-bonds prevent N-glycosylation at some sites.
    N-glycosylated.
  • Cellular localizationCell membrane. Secreted. A secreted form also exists.
  • Information by UniProt
  • Alternative names
    • C-type lectin domain family 8 member A
    • CLEC8A
    • hLOX-1
    • Lectin-like oxidized LDL receptor 1
    • Lectin-like oxLDL receptor 1
    • Lectin-type oxidized LDL receptor 1
    • LOX-1
    • LOXIN
    • Olr1
    • OLR1_HUMAN
    • Ox-LDL receptor 1
    • Oxidized low density lipoprotein (lectin like) receptor 1
    • Oxidized low density lipoprotein receptor 1 soluble form
    • Oxidized low-density lipoprotein receptor 1
    • SCARE1
    • Scavenger receptor class E member 1
    • SLOX1
    • soluble form
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

LOX1 (OLR1) Mouse ELISA Kit images

  • Representative Standard Curve using ab119603

Protocols

References for LOX1 (OLR1) Mouse ELISA Kit (ab119603)

ab119603 has not yet been referenced specifically in any publications.

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