Overview

  • Product nameAnti-LOXL1 antibody
    See all LOXL1 primary antibodies
  • Description
    Rabbit polyclonal to LOXL1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human LOXL1.

    (Peptide available as ab97928.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Heart; Spinal Cord; Colon.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 63 kDa).

Target

  • RelevanceLOXL1 is a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL1 is active on elastin and collagen substrates. Genetic variations in LOXL1 are associated with risk of developing exfoliation syndrome (XFS) [MIM:177650]; also called exfoliation glaucoma (XFG). Exfoliation syndrome (XFS) is characterized by accumulation of abnormal microfibrillar deposits that line the aqueous bathed surfaces of the anterior segment of the eye. The prevalence of XFS increases with age, and a number of studies have pointed to a geographical clustering of XFS, although this condition is found worldwide; reported prevalence rates average about 10 to 20% of the general population over age 60.
  • Cellular localizationSecreted, extracellular space.
  • Database links
  • Alternative names
    • Lysyl oxidase like 1 antibody
    • LOL antibody
    • LOXL antibody
    • Lysyl oxidase homolog 1 antibody
    • Lysyl oxidase like protein 1 antibody
    see all

Anti-LOXL1 antibody images

  • All lanes : Anti-LOXL1 antibody (ab87748) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 3 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-LOXL1 antibody (ab87748)

ab87748 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products.

I can confirm that ab97928 LOXL1 peptide was used to raise the antibody against LOXL1 (ab87748) and it can be used as blocking peptide with studies using http://www.abcam.com/index...

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I apologize for being late in getting back to you.
The peptide ab97928 is in stock. You can place an order any time.
I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"