Overview

  • Product name
  • Description
    Rabbit polyclonal to LOXL2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LOXL2.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: LNCaP; DU145; A431; A549; MDA MB 231; MCF7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).

Target

  • Tissue specificity
    Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • Sequence similarities
    Belongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications
    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Images

  • All lanes : Anti-LOXL2 antibody (ab113201) at 1 µg/ml

    Lane 1 : LNCaP human prostate carcinoma cell line lysate Whole Cell Lysate
    Lane 2 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 86 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,86 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds

References

ab113201 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab113201.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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