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Rabbit polyclonal to LOXL2
Predicted to work with:
Horse, Macaque monkey, Gorilla, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LOXL2.
This antibody gave a positive signal in the following whole cell lysates: LNCaP; DU145; A431; A549; MDA MB 231; MCF7.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
Belongs to the lysyl oxidase family.
Contains 4 SRCR domains.
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Secreted > extracellular space.
Information by UniProt
LOR 2 antibody
LOX L2 antibody
Western blot - Anti-LOXL2 antibody (ab113201)
All lanes :
Anti-LOXL2 antibody (ab113201) at 1 µg/ml
Lane 1 :
LNCaP human prostate carcinoma cell line lysate Whole Cell Lysate
Lane 2 :
DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 3 :
A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 :
A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 :
MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 :
MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
90 kDa (
why is the actual band size different from the predicted?
Additional bands at:
35 kDa, 86 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
LOXL2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The band observed at 86 kDa could potentially be a cleaved form of LOXL2 due to the presence of a 25 amino acid signal peptide.
has not yet been referenced specifically in any publications.
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