Overview

  • Product nameAnti-LOXL2 antibody
    See all LOXL2 primary antibodies
  • Description
    Mouse monoclonal to LOXL2
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment with tag:

    NFGDQGITMGCWDMYRHDIDCQWVDITDVPPGDYLFQVVINPNFEVAESD YSNNIMKCRSRYDGHRIWMYNCHIGGSFSEETEKKFEHFSGLLNNQLSP

    corresponding to amino acids 675-774 of Human LOXL2

Properties

Applications

Our Abpromise guarantee covers the use of ab55470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 86 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen and transfected lysates. We have no data on the detection of endogenous protein.
ChIP Use at an assay dependent concentration.

Target

  • Tissue specificityExpressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • Sequence similaritiesBelongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications
    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Anti-LOXL2 antibody images



  • Predicted band size : 86 kDa
  • ChIP analysis of mouse embryonic fibroblasts using ab55470 to bind LOXL2.

    Chromatin was obtained by cross-linking with 1% formaldehyde at 37°C for 10 minutes. The primary antibody was used at 1/500 dilution for 16 hours at 4°C. Protein binding was detected using real-time PCR.

    See Abreview

References for Anti-LOXL2 antibody (ab55470)

This product has been referenced in:
  • Millanes-Romero A  et al. Regulation of heterochromatin transcription by Snail1/LOXL2 during epithelial-to-mesenchymal transition. Mol Cell 52:746-57 (2013). Read more (PubMed: 24239292) »

See 1 Publication for this product

Product Wall

Abreviews
Application ChIP
Sample Mouse Cell lysate - whole cell (MEFs)
Specification MEFs
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1 % (37ºC)
Detection step Real-time PCR
Positive control Experiment previously done with an unavailable antibody from ABCAM (ab60753)
Username

Abcam user community

Verified customer

Submitted Jan 21 2013

Application Western blot
Sample Human Cell lysate - whole cell (human MEFs)
Loading amount 40 µg
Specification human MEFs
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

Submitted Dec 04 2012

Gracias por tu interés en nuestro sistema Abreview.

He creado un código de descuento: XXXX para testar ab55470 en ChIP.

El código caduca el día XXXXX

Este código es canjeable por un anticu...

Read More

Gracias por contactarnos.

Lamento mucho que el producto que os interesa haya sido descatalogado. El motivo para ello es que el colaborador que nos lo proporcionaba ha dejado de fabricarlo.

La alternativa sugerida es un buen anticuer...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (MDA MB231 WT condition media)
Total protein in input 70 µg
Specification MDA MB231 WT condition media
Immuno-precipitation step Protein G
Username

Miss. Joan Chang

Verified customer

Submitted Jun 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"