Overview

  • Product nameAnti-LOXL2 antibody
    See all LOXL2 primary antibodies
  • Description
    Rabbit polyclonal to LOXL2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 454-712 of Human LOXL2 (NP_002309).

  • Positive control
    • 293T, A431 and H1299 whole cell lysates; HeLa, HepG2, Molt-4 and Raji lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • Tissue specificityExpressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
  • Sequence similaritiesBelongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications
    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOR 2 antibody
    • LOR2 antibody
    • LOX L2 antibody
    • LOXL 2 antibody
    • LOXL2 antibody
    • LOXL2_HUMAN antibody
    • Lysyl oxidase homolog 2 antibody
    • Lysyl oxidase like 2 antibody
    • Lysyl oxidase like protein 2 antibody
    • Lysyl oxidase related 2 antibody
    • Lysyl oxidase related protein 2 antibody
    • Lysyl oxidase related protein WS9 14 antibody
    • Lysyl oxidase-like protein 2 antibody
    • Lysyl oxidase-related protein 2 antibody
    • Lysyl oxidase-related protein WS9-14 antibody
    • WS9 14 antibody
    see all

Anti-LOXL2 antibody images

  • All lanes : Anti-LOXL2 antibody (ab96233) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : H1299 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 87 kDa
  • IHC image of ab96233 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96233, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-LOXL2 antibody (ab96233)

This product has been referenced in:
  • Mammoto A  et al. Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics. Nat Commun 4:1759 (2013). Read more (PubMed: 23612300) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Other
Sample Human Tissue sections (human cartilage)
Specification human cartilage
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
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Submitted Apr 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0, microwave 65c
Sample Mouse Tissue sections (mouse knee joint)
Specification mouse knee joint
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 18 2014

These were lysates of non-transfected cells, so the antibody is detecting native LOXL2.

Thank you for your patience. IgG ab27472 LOT: GR49002-1 I can confirm that the concentration is 2 ug/ml. Please do not hesitate to contact me with additional questions or concerns.

Thank you for your enquiry and your interest. s-SMA ab15734 LOT: GR17115-1 concentration: 0.3 mg/ml LOX ab31238 LOT: GR13790-1 concentration: 1 mg/ml LOXL2 ab96233 LOT: GR6538-6 concentration: 1 mg/ml I am contacting the Lab to find out the...

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Thank you for your enquiry and interest. Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should mat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"