Recombinant
RabMAb

Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810)

Overview

  • Product name
    Anti-LOXL2 antibody [EPR12733] - C-terminal
    See all LOXL2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12733] to LOXL2 - C-terminal
  • Specificity
    Our lab has been unable to use this product successfully in IHC-P and so we cannot guarantee that it will work in this application. However, some customers have been successful with this antibody in IHC-P.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LOXL2 aa 750 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y4K0

  • Positive control
    • WB: MCF-7, MDA-MB-435, A431, U87-MG
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 87 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity
      Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate.
    • Sequence similarities
      Belongs to the lysyl oxidase family.
      Contains 4 SRCR domains.
    • Post-translational
      modifications
      The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • LOR 2 antibody
      • LOR2 antibody
      • LOX L2 antibody
      • LOXL 2 antibody
      • LOXL2 antibody
      • LOXL2_HUMAN antibody
      • Lysyl oxidase homolog 2 antibody
      • Lysyl oxidase like 2 antibody
      • Lysyl oxidase like protein 2 antibody
      • Lysyl oxidase related 2 antibody
      • Lysyl oxidase related protein 2 antibody
      • Lysyl oxidase related protein WS9 14 antibody
      • Lysyl oxidase-like protein 2 antibody
      • Lysyl oxidase-related protein 2 antibody
      • Lysyl oxidase-related protein WS9-14 antibody
      • WS9 14 antibody
      see all

    Images

    • Lanes 1, 2 & 4 : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/1000 dilution
      Lane 3 : Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/200 dilution

      Lane 1 : MCF-7 (Human breast carcinoma) whole cell lysate
      Lane 2 : MDA-MB-435 (Human ductal carcinoma) whole cell lysate
      Lane 3 : A431 (Human epidermoid carcinoma) whole cell lysate
      Lane 4 : U87-MG (Human glioblastoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 87 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)

      Exposure time:

      Lane 1-3: 3min
      Lane 4: 1min

      Blocking/Diluting buffer and concentration 5% NFDM /TBST

      MCF-7 lack LOXL2 expression (PMID: 19330836, PMID:12154058 and PMID:  27655685)

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, adult)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, adult
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Oct 12 2017

    This migration pattern is actually common for LOXL2 as demonstrated by Hollosi et al in their article "Lysyl oxidase-like 2 promotes migration in noninvasive breast cancer cells but not in normal breast epithelial cells" (2009).

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Other
    Sample
    Mouse Tissue sections (mouse knee)
    Specification
    mouse knee
    Permeabilization
    Yes - 0.2% Triton X-100 and PBS
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 31 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up