Overview

  • Product nameAnti-LOXL4 antibody
    See all LOXL4 primary antibodies
  • Description
    Rabbit polyclonal to LOXL4
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LOXL4.

    (Peptide available as ab99480.)

  • Positive control
    • This antibody gave a positive signal in Human Testis and Skin Tissue Lysates. IF/ICC: A549 cell line.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 84 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMay modulate the formation of a collagenous extracellular matrix.
  • Tissue specificityExpressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage.
  • Sequence similaritiesBelongs to the lysyl oxidase family.
    Contains 4 SRCR domains.
  • Post-translational
    modifications
    The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4833426I20Rik antibody
    • FLJ21889 antibody
    • LOX L4 antibody
    • LOXC antibody
    • LOXL 4 antibody
    • LOXL4 antibody
    • LOXL4_HUMAN antibody
    • Lysyl oxidase homolog 4 antibody
    • Lysyl oxidase like 4 antibody
    • Lysyl oxidase like protein 4 antibody
    • Lysyl oxidase related C antibody
    • Lysyl oxidase related protein C antibody
    • Lysyl oxidase-like protein 4 antibody
    • Lysyl oxidase-related protein C antibody
    see all

Anti-LOXL4 antibody images

  • All lanes : Anti-LOXL4 antibody (ab88186) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes
    The band observed at 80 kDa could potentially be a cleaved form of LOXL4. Abcam welcomes customer feedback.
  • IHC image of ab88186 staining in human testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88186, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab88186 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88186, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-LOXL4 antibody (ab88186)

This product has been referenced in:
  • Jiang WP  et al. Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis. Int J Oral Sci 6:31-8 (2014). WB, IHC-P ; Human . Read more (PubMed: 24357854) »

See 1 Publication for this product

Product Wall

Thank you for your email. I have emailed the lab regarding the blocking experiment. I will let you know what I hear back. The immunogen peptide is available for sale as ab99480. Regarding the imunogen sequence, I would need to kno...

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Thank you for contacting us. I have contacted the lab to check if experiments have been performed to check cross reactivity, but I can say from checking the homology for the immunogen with LOXL3 that there is only 50% homology with lots of gaps...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"