• Product nameAnti-LPCAT1 antibody
    See all LPCAT1 primary antibodies
  • Description
    Rabbit polyclonal to LPCAT1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to an internal region within amino acids 324-373 (


    ) of Human LPCAT1 (NP_079106)

  • Positive control
    • Human fetal small intestine lysate IF/ICC: A549 cell line.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG


Our Abpromise guarantee covers the use of ab94903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionPossesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology.
  • PathwayLipid metabolism; phospholipid metabolism.
  • Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    Contains 2 EF-hand domains.
  • DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.
    The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 acylglycerophosphocholine O acyltransferase antibody
    • 1 alkylglycerophosphocholine O acetyltransferase antibody
    • 1-acylglycerophosphocholine O-acyltransferase antibody
    • 1-alkylglycerophosphocholine O-acetyltransferase antibody
    • Acetyl CoA:lyso PAF acetyltransferase antibody
    • Acetyl CoA:lyso platelet activating factor acetyltransferase antibody
    • Acetyl-CoA:lyso-PAF acetyltransferase antibody
    • Acetyl-CoA:lyso-platelet-activating factor acetyltransferase antibody
    • Acyl CoA:lysophosphatidylcholine acyltransferase 1 antibody
    • Acyltransferase like 2 antibody
    • Acyltransferase-like 2 antibody
    • AGPAT10 antibody
    • AGPAT9 antibody
    • AYTL1 antibody
    • AYTL2 antibody
    • FLJ12443 antibody
    • FLJ41609 antibody
    • LPC acyltransferase 1 antibody
    • LPC acyltransferase antibody
    • lpcat antibody
    • LPCAT-1 antibody
    • LPCAT1 antibody
    • Lyso PAF acetyltransferase antibody
    • Lyso-PAF acetyltransferase antibody
    • LysoPAFAT antibody
    • LysoPC acyltransferase 1 antibody
    • Lysophosphatidylcholine acyltransferase 1 antibody
    • PCAT1_HUMAN antibody
    • PFAAP3 antibody
    • Phosphonoformate immuno associated protein 3 antibody
    • Phosphonoformate immuno-associated protein 3 antibody
    see all

Anti-LPCAT1 antibody images

  • ICC/IF image of ab94903 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94903, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-LPCAT1 antibody (ab94903) at 1 µg/ml + Human fetal small intestine lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa

References for Anti-LPCAT1 antibody (ab94903)

ab94903 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

Regarding a positive control, I would like to recommend the following:

ab29277 - Small intestine (Human) Whole Cell Lysate - fetal normal tissue
ab30203 - Kidney (Human) Tissue Lysate - adult normal ...

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Thank you very much for your interest in ab94903.

To our knowledge, ab94903 has not been tested in rat in WB. Therefore, I can offer a discount off a future purchase if you buy ab94903 now, test it in rat in WB and submit feedback to us in t...

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