Overview

  • Product name
    Anti-LRCH1 antibody
  • Description
    Rabbit polyclonal to LRCH1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 310-421 of Human LRCH1.

  • Positive control
    • Human hippocampus tissue; RT 4, U 251 MG, Human Liver, and Human Tonsil lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Sequence similarities
    Contains 1 CH (calponin-homology) domain.
    Contains 9 LRR (leucine-rich) repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calponin homology domain-containing protein 1 antibody
    • CHDC1 antibody
    • KIAA1016 antibody
    • Leucine-rich repeat and calponin homology domain-containing protein 1 antibody
    • Lrch1 antibody
    • LRCH1_HUMAN antibody
    • Neuronal protein 81 antibody
    • NP81 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus, nucleoli, cytoplasm and cytoskeleton (actin filaments). Recommended concentration of ab121679 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121679 at 1/400 dilution staining LRCH1 in Paraffin-embedded Human hippocampus tissue by Immunohistochemistry.
  • All lanes : Anti-LRCH1 antibody (ab121679) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab121679 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab121679.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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