Overview

  • Product name
    Anti-LRFN5 antibody
  • Description
    Rabbit polyclonal to LRFN5
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 16 aa from near the C-terminus of Human LRFN5 (Q96NI6).

  • Positive control
    • EL4 cell lysate, Mouse brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 79 kDa.

Target

  • Function
    Cell adhesion molecule that mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Promotes neurite outgrowth in hippocampal neurons.
  • Sequence similarities
    Belongs to the LRFN family.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Domain
    Lacks a cytoplasmic PDZ-binding motif, which has been implicated in function of related LRFN proteins.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf146 antibody
    • DKFZp686G0210 antibody
    • Fibronectin type III immunoglobulin and leucine rich repeat domains 8 antibody
    • FIGLER8 antibody
    • FLJ30803 antibody
    • leucine rich repeat and fibronectin type III domain containing 5 antibody
    • Leucine-rich repeat and fibronectin type-III domain-containing protein 5 antibody
    • Lrfn5 antibody
    • LRFN5_HUMAN antibody
    • SALM5 antibody
    see all

Images

  • Lane 1 : Anti-LRFN5 antibody (ab106370) at 0.5 µg/ml
    Lane 2 : Anti-LRFN5 antibody (ab106370) at 1 µg/ml

    Lane 1 : EL4 cell lysate
    Lane 2 : EL4 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 79 kDa
  • ab106370, at 5 ug/ml, staining LRFN5 in Mouse brain tissue by immunohistochemistry.

References

This product has been referenced in:
  • Burch A  et al. A novel synaptic junction preparation for the identification and characterization of cleft proteins. PLoS One 12:e0174895 (2017). Read more (PubMed: 28362857) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your reply.

The discount code is valid for untested species / applications. Western Blot is a tested application for the three of them, so I am afraid the discount code would not be applicable in these cases. You can still send ...

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Thank you for contacting us.

Ab106370 has been used in WB and IHC with paraffin embedded sections in Mouse, Rat and Human species. Ab105169 has successfully worked in WB and IHC in both frozen and paraffin embedded sections and ab87165 has on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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