The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 µg/ml, performed on lysates from over-expressing cells. Predicted molecular weight: 120 kDa.
LRIG1 protein migrates during denaturing polyacrylamide gel electrophoresis under reducing conditions as two species with apparent molecular weights of 143kDa and 134kDa, and as two fragments corresponding to an N-terminal 111kDa species and a C-terminal 32kDa species. Under non-reducing conditions, both apparent monomers and apparent higher molecular weight complexes were evident.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAct as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.