Anti-LRP1 antibody [5A6] (ab28320)

  Immunocytochemistry/ Immunofluorescence - Low Density LRP antibody [5A6] (ab28320)

ab28320 at 1/100 staining human HUVEC cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incuabtion with the antibody for 1 hour. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

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  Western blot - Low Density LRP antibody [5A6] (ab28320)

ab28320 at 1/3000 + WI38 fibroblasts whole cell lysate

Secondary
HRP conjugated donkey anti-mouse polyclonal diluted 1/10000
developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 85 kDa
Observed band size : 85 kDa


Exposure time : 2 minutes

50µg of human fibroblast whole cell lysate were loaded on to the gel and run under denaturing conditions.

The membrane was blocked with 1% BSA for 1 hour at RT.

The primary antibody was incubated for 12 hours at 4°C.

This image is courtesy of an Abreview submitted by Dr Torry Tucker

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  Flow Cytometry - Low Density LRP antibody [5A6] (ab28320)

Overlay histogram showing HepG2 cells stained with ab28320 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28320, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.