• Product name
    Anti-LRP1 antibody [8G1]
    See all LRP1 primary antibodies
  • Description
    Mouse monoclonal [8G1] to LRP1
  • Specificity
    Specifically reacts with the 515kDa alpha chain of LRP/alpha2 MR expressed in hepatocytes, tissue macrophages, subsets of neurons and astrocytes in the CNS, fibroblasts, smooth muscle cells and monocyte derived foam cells in atherosclerotic lesions of arterial walls.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, ICC/IF, Electron Microscopy, Dot blot, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to LRP1. J Biol Chem 265:17401-4 (1990).


Our Abpromise guarantee covers the use of ab20384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Use under non reducing condition.
ICC/IF Use a concentration of 5 µg/ml.
Electron Microscopy Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins.
Dot blot Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function
    Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
    Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
  • Tissue specificity
    Most abundant in liver, brain and lung.
  • Sequence similarities
    Belongs to the LDLR family.
    Contains 22 EGF-like domains.
    Contains 31 LDL-receptor class A domains.
    Contains 34 LDL-receptor class B repeats.
  • Post-translational
    Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
    The N-terminus is blocked.
    Phosphorylated on serine and threonine residues.
    Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
  • Cellular localization
    Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2MR antibody
    • Alpha 2 macroglobulin receptor antibody
    • alpha 2MR antibody
    • Alpha-2-macroglobulin receptor antibody
    • APOER antibody
    • Apolipoprotein E receptor antibody
    • APR antibody
    • CD 91 antibody
    • CD91 antibody
    • CD91 antigen antibody
    • IGFBP3R antibody
    • LDL receptor related protein 1 antibody
    • Low density lipoprotein receptor related protein 1 antibody
    • Low density lipoprotein related protein 1 antibody
    • Low-density lipoprotein receptor-related protein 1 intracellular domain antibody
    • LRP 1 antibody
    • LRP 515 antibody
    • LRP 85 antibody
    • LRP antibody
    • LRP ICD antibody
    • LRP-1 antibody
    • LRP-515 antibody
    • LRP-85 antibody
    • Lrp1 antibody
    • LRP1 protein antibody
    • LRP1_HUMAN antibody
    • LRP1A antibody
    • LRP515 antibody
    • LRP85 antibody
    • LRPICD antibody
    • MGC88725 antibody
    • Prolow density lipoprotein receptor related protein 1 antibody
    • TbetaR V/LRP 1/IGFBP 3 receptor antibody
    • TbetaRV/LRP1/IGFBP3 receptor antibody
    • TGFBR 5 antibody
    • TGFBR5 antibody
    • Type V tgf beta receptor antibody
    see all


  • ab20384 staining human mesothelial cells by flow cytometry.  Cells were lifted in lifting buffer plus FBS and fixed with formaldehyde.  The cell population was gated against mIgG control.  The primary antibody was diluted 1/100 in 0.2% BSA in PBS and incubated with the sample at a concentration of 1 µg/100 µl for 1 hour at 4°C.  A FITC conjugated goat anti-mouse antibody was used as the secondary, diluted 1/100 and incubated with the sample at a concentration of 1 µg/100 µl.

    See Abreview

  • Ab20384 staining primary rat hepatocytes cells by EM. The cells were fixed and prepared by Tokuyasu method. The cryosections of 70nm were placed on 100 mesh grids. All the following steps were done at room temperature. Blocking was carried out for 30 min with 1% fish skin gelatine (FSG). The tissue sections were placed on a drop of 5 ul of first Ab (anti-LRP 8G1) for 20 min at dilutions of 1:400 or 1:600 seemed to work the best. After incubation with the primary antibody, 3. 5 x 2 min wash was done with PBS and the sections were placed on a drop of 5 ul of secondary Ab (goat anti-mouse) for 20 min (dilution 1:150 5. 5 x 2 min wash with PBS). Samples were placed on a drop of 5 ul of PAG10 for 15 min (dilution 1:60 or 1:70) and then 5 x 2 min wash with PBS. 2 min on 1% glutaraldehyde for fixation and 4 x 2.5 min on H2O. afterthat 1 second + 1 second + 6 min on drops of Methyl Cellulose/Uranyl Acetate (9:1) on ice. The surplus of MC/UA was removed , the slides were dried and observed at the TEM. This image was kindly provided by our customer.
  • ICC/IF image of ab20384 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20384, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Dohler F  et al. High molecular mass assemblies of amyloid-ß oligomers bind prion protein in patients with Alzheimer's disease. Brain 137:873-86 (2014). WB ; Human . Read more (PubMed: 24519981) »
  • Scilabra SD  et al. Differential regulation of extracellular tissue inhibitor of metalloproteinases-3 levels by cell membrane-bound and shed low density lipoprotein receptor-related protein 1. J Biol Chem 288:332-42 (2013). WB . Read more (PubMed: 23166318) »

See all 5 Publications for this product

Customer reviews and Q&As

I checked the LRP1 antibodies and ab20384 has 93% homology with C. elegans.

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For ab20384, the lab recommends using human umbilical vein endothelial cells (HUVEC) as a negative control. If you do not have access to this type of cells, according to the human protein atlas (linked below),Low ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Human Cell (Mesothelial Cels)
Mesothelial Cels
Gating Strategy
gated against mIgG control

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Verified customer

Submitted Apr 04 2008


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