Anti-LRP1 antibody (ab168454)
Key features and details
- Mouse polyclonal to LRP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LRP1 antibody
See all LRP1 primary antibodies -
Description
Mouse polyclonal to LRP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to amino acids 1-292 of Human LRP1. (AAH45107.1)
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Positive control
- Transfected 293T cell lysate; Human endometrium tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa.
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IHC-P |
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa. |
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
Tissue specificity
Most abundant in liver, brain and lung. -
Sequence similarities
Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
Post-translational
modificationsCleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
Cellular localization
Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit. - Information by UniProt
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Database links
- Entrez Gene: 4035 Human
- Entrez Gene: 16971 Mouse
- Omim: 107770 Human
- SwissProt: Q07954 Human
- SwissProt: Q91ZX7 Mouse
- Unigene: 162757 Human
- Unigene: 271854 Mouse
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Alternative names
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
Images
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All lanes : Anti-LRP1 antibody (ab168454) at 1 µg/ml
Lane 1 : LRP1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-mouse HRP at 1/2500 dilution -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LRP1 with ab168454 at 10µg/ml.
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Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Human endometrium tissue labeling LRP1 with ab168454 at 3µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab168454 has been referenced in 2 publications.
- Zheng Y et al. Anti-PAI-1 Monoclonal Antibody Inhibits the Metastasis and Growth of Esophageal Squamous Cell Carcinoma. J Cancer 14:114-128 (2023). PubMed: 36605486
- Ma J et al. Neutrophil elastase-regulated macrophage sheddome/secretome and phagocytic failure. Am J Physiol Lung Cell Mol Physiol 321:L555-L565 (2021). PubMed: 34261337