Overview

  • Product nameAnti-LRP11 antibody
    See all LRP11 primary antibodies
  • Description
    Rabbit polyclonal to LRP11
  • Specificityab74099 detects endogenous levels of total LRP11 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal sequence of human LRP11.

  • Positive control
    • Extracts from Jurkat cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.
ELISA 1/40000.

Target

  • RelevanceLRP11 belongs to the LDLR family. It contains one LDL-receptor class A domain, one MANEC domain and one PKD domain. There are two named isoforms.
  • Cellular localizationMembrane; Single pass type I membrane protein
  • Database links
  • Alternative names
    • bA350J20.3 antibody
    • Low density lipoprotein receptor related protein 11 antibody
    • LRP 11 antibody
    • MANSC3 antibody

Anti-LRP11 antibody images

  • All lanes : Anti-LRP11 antibody (ab74099) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells with no peptide
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
  • LRP11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to LRP11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74099.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 53kDa; LRP11

References for Anti-LRP11 antibody (ab74099)

ab74099 has not yet been referenced specifically in any publications.

Product Wall

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The peptide has been added to the catalog as ab136161.
http://www.abcam.com/index.html?datasheet=136161

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If you could, please remind me if you were interested in receiving another vial of ab43870 or if youwould likea different primary antibody. Please let me know what the catalog number w...

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DISCOUNT CODE: xxx for ab74099
DISCOUNT CODE: xxx for ab69212
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab74099 and ab69212 in Functional stu...

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Thank you for your patience. I have received further information for these 2 antibodies: ab65813: The homology is 84.6%. Thus, crossreactivity with mouse might be likely. ab74099: homology is 64.3%. Mouse tissues have not yet been tested with ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"