Anti-LRP4 antibody (ab85697)
Key features and details
- Goat polyclonal to LRP4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LRP4 antibody
See all LRP4 primary antibodies -
Description
Goat polyclonal to LRP4 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide:
C-SNPSYRTSTQEVK
, corresponding to internal sequence amino acids 1765-1777 of Human LRP4 (NP_002325.2). -
Positive control
- Human brain (cerebral cortex) lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85697 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 216 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes. Involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. -
Tissue specificity
Expressed in several regions of the brain. -
Involvement in disease
Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]. It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. -
Sequence similarities
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 20 LDL-receptor class B repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 504317 Cow
- Entrez Gene: 4038 Human
- Entrez Gene: 228357 Mouse
- Entrez Gene: 83469 Rat
- Omim: 604270 Human
- SwissProt: O75096 Human
- SwissProt: Q8VI56 Mouse
- SwissProt: Q9Z319 Mouse
see all -
Alternative names
- KIAA0816 antibody
- LDLR dan antibody
- Low density lipoprotein receptor related protein 4 antibody
see all
Images
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Anti-LRP4 antibody (ab85697) at 0.1 µg/ml + Human cerebral cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 216 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (3)
ab85697 has been referenced in 3 publications.
- Ohkawara B et al. CTGF/CCN2 facilitates LRP4-mediated formation of the embryonic neuromuscular junction. EMBO Rep 21:e48462 (2020). PubMed: 32558157
- Selcen D et al. Impaired Synaptic Development, Maintenance, and Neuromuscular Transmission in LRP4-Related Myasthenia. JAMA Neurol 72:889-96 (2015). PubMed: 26052878
- Ohkawara B et al. LRP4 third ß-propeller domain mutations cause novel congenital myasthenia by compromising agrin-mediated MuSK signaling in a position-specific manner. Hum Mol Genet N/A:N/A (2013). PubMed: 24234652