Overview

  • Product nameAnti-LRP5L antibody
    See all LRP5L primary antibodies
  • Description
    Rabbit polyclonal to LRP5L
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    amino acids 198-251 (VIIDQLPDLM GLKAVNVDKV VGTNPHADRN GGAATCASSR PTQPGLAAPS RAWN) of Human LRP5L (A4QPB2).

  • Positive control
    • HEK293T transfected lysate. Human skeletal muscle tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-LRP5L antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122013 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-LRP5L antibody (ab122013) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : LRP5L (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate

    Developed using the ECL technique
  • ab122013, at a 1/10 dilution, staining Human LRP5L in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed parraffin-embedded tissue. Moderate cytoplasmic positivity in myocytes shown.

References for Anti-LRP5L antibody (ab122013)

ab122013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"