Overview

  • Product name
  • Description
    Rabbit polyclonal to LRP5L
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    amino acids 198-251 (VIIDQLPDLM GLKAVNVDKV VGTNPHADRN GGAATCASSR PTQPGLAAPS RAWN) of Human LRP5L (A4QPB2).

  • Positive control
    • HEK293T transfected lysate. Human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122013 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-LRP5L antibody (ab122013) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : LRP5L (co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) transfected HEK293T lysate

    Developed using the ECL technique
  • ab122013, at a 1/10 dilution, staining Human LRP5L in skeletal muscle, using Immunohistochemistry, Formalin/PFA-fixed parraffin-embedded tissue. Moderate cytoplasmic positivity in myocytes shown.

References

ab122013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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