Anti-LRPAP1 antibody [7F1] (ab20368)

Overview

  • Product nameAnti-LRPAP1 antibody [7F1]
    See all LRPAP1 primary antibodies
  • Description
    Mouse monoclonal [7F1] to LRPAP1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rabbit
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab20368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa.

Target

  • FunctionInteracts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
  • Involvement in diseaseNote=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
  • Sequence similaritiesBelongs to the alpha-2-MRAP family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationEndoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39 kDa receptor-associated protein antibody
    • A2MRAP antibody
    • A2RAP antibody
    • Alpha 2 macroglobulin receptor associated protein antibody
    • Alpha 2 MRAP antibody
    • Alpha-2-macroglobulin receptor-associated protein antibody
    • Alpha-2-MRAP antibody
    • AMRP_HUMAN antibody
    • HBP44 antibody
    • Lipoprotein receptor associated protein antibody
    • Low density lipoprotein receptor related protein associated protein 1 antibody
    • Low density lipoprotein receptor-related protein-associated protein 1 antibody
    • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
    • Low density lipoprotein related protein associated protein 1 antibody
    • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
    • Lrpap1 antibody
    • MGC138272 antibody
    • MRAP antibody
    • MYP23 antibody
    • RAP antibody
    see all

Anti-LRPAP1 antibody [7F1] images

  • ICC/IF image of ab20368 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20368, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-LRPAP1 antibody [7F1] (ab20368)

ab20368 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your enquiry. You are right, the datasheet for this particular antibody. Ab20368 recognizes the 39 kDa low density lipoprotein receptor-related protein-associated protein 1 (RAP), not RAP1. We are in the process of changing t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"