Overview

  • Product name
  • Description
    Rabbit polyclonal to LRPAP1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human LRPAP1.

  • Positive control
    • This antibody gave a positive signal in the following lysates: HEK293 Whole Cell; A498 Whole Cell; Human Brain Tissue; U-87 MG Whole Cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab85591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Function
    Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
  • Involvement in disease
    Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
  • Sequence similarities
    Belongs to the alpha-2-MRAP family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39 kDa receptor-associated protein antibody
    • A2MRAP antibody
    • A2RAP antibody
    • Alpha 2 macroglobulin receptor associated protein antibody
    • Alpha 2 MRAP antibody
    • Alpha-2-macroglobulin receptor-associated protein antibody
    • Alpha-2-MRAP antibody
    • AMRP_HUMAN antibody
    • HBP44 antibody
    • Lipoprotein receptor associated protein antibody
    • Low density lipoprotein receptor related protein associated protein 1 antibody
    • Low density lipoprotein receptor-related protein-associated protein 1 antibody
    • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
    • Low density lipoprotein related protein associated protein 1 antibody
    • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
    • Lrpap1 antibody
    • MGC138272 antibody
    • MRAP antibody
    • MYP23 antibody
    • RAP antibody
    see all

Images

  • All lanes : Anti-LRPAP1 antibody (ab85591) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 37 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab85591 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85591, µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa cells at 5µg/ml.
  • IHC image of LRPAP1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85591, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

ab85591 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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