Anti-LRPAP1 antibody [EPR3327] (ab76500)

Overview

  • Product nameAnti-LRPAP1 antibody [EPR3327]
    See all LRPAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3327] to LRPAP1
  • Tested applicationsSuitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human LRPAP1 aa 1-100.

  • Positive control
    • WB: Human fetal kidney, mouse kidney and rat kidney tissue lysate and 293T cell lysates. IHC-P: Human hepatocellular carcinoma and brain tissues. IP: Human fetal kidney tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50 - 1/200.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionInteracts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
    • Involvement in diseaseNote=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
    • Sequence similaritiesBelongs to the alpha-2-MRAP family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationEndoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • 39 kDa receptor-associated protein antibody
      • A2MRAP antibody
      • A2RAP antibody
      • Alpha 2 macroglobulin receptor associated protein antibody
      • Alpha 2 MRAP antibody
      • Alpha-2-macroglobulin receptor-associated protein antibody
      • Alpha-2-MRAP antibody
      • AMRP_HUMAN antibody
      • HBP44 antibody
      • Lipoprotein receptor associated protein antibody
      • Low density lipoprotein receptor related protein associated protein 1 antibody
      • Low density lipoprotein receptor-related protein-associated protein 1 antibody
      • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
      • Low density lipoprotein related protein associated protein 1 antibody
      • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
      • Lrpap1 antibody
      • MGC138272 antibody
      • MRAP antibody
      • MYP23 antibody
      • RAP antibody
      see all

    Anti-LRPAP1 antibody [EPR3327] images

    • All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/6000 dilution (unpurified)

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Rat kidney tissue lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 41 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST

    • All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/3000 dilution (purified)

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Rat kidney tissue lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 41 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with unpurified ab76500 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with purified ab76500 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling LRPAP1 with unpurified ab76500 at a 1/100 dilution.

    • ab76500 (unpurified) at 1/300 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • ab76500 (purified) at 1/150 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-LRPAP1 antibody [EPR3327] (ab76500)

    ab76500 has not yet been referenced specifically in any publications.

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