Overview

  • Product nameAnti-LRPPRC antibody
    See all LRPPRC primary antibodies
  • Description
    Rabbit polyclonal to LRPPRC
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 611-907 of Human LRPPRC (NP_573566)

  • Positive control
    • H1299, HeLa, 293T, A431, HepG2, Molt-4 and Raji cell lines. SW480 Xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab97505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 158 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.
  • Tissue specificityExpressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.
  • Involvement in diseaseLeigh syndrome French-Canadian type
  • Sequence similaritiesContains 20 PPR (pentatricopeptide) repeats.
  • Cellular localizationMitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa leucine-rich protein antibody
    • gp130 antibody
    • Leucine-rich PPR motif-containing protein antibody
    • Leucine-rich PPR-motif containing protein antibody
    • LPPRC_HUMAN antibody
    • LRP 130 antibody
    • lrpprc antibody
    • mitochondrial antibody
    see all

Anti-LRPPRC antibody images

  • All lanes : Anti-LRPPRC antibody (ab97505) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 158 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97505 at 1/200 dilution. Lower panel is also merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using ab97505 at 1/100 dilution.

References for Anti-LRPPRC antibody (ab97505)

This product has been referenced in:
  • Edgett BA  et al. The effect of acute and chronic sprint-interval training on LRP130, SIRT3, and PGC-1a expression in human skeletal muscle. Physiol Rep 4:N/A (2016). WB . Read more (PubMed: 27604398) »
  • Clemente P  et al. hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria. J Biol Chem 288:8321-31 (2013). Read more (PubMed: 23362268) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions Reduced Denaturing (6% Gel)
Loading amount 3.25 µg
Specification Skeletal Muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Nov 26 2015

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I am sending this email to inquire about the updates you might have about the WB analysis. There were few anti KPNA4 products that were proving difficult to use and as per your last email you were suppose to contact m...

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We have 3 antibodies ab6035, ab6039 and ab82149 available from the same collaborator as for ab6038, and ab6036. It is hard to guess if these 3 antibodies will perform as good as ab6038/ ab6036 antibodies or even ...

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Thank you very much for filling the questionnaires. I am glad to hear that the antibody ab97505 is working in retest experiment. Because you have not mentioned ab88859 so I am assuming that this antibody is also successful.

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