Anti-LRPPRC/GP130 antibody (ab97505)
Key features and details
- Rabbit polyclonal to LRPPRC/GP130
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LRPPRC/GP130 antibody
See all LRPPRC/GP130 primary antibodies -
Description
Rabbit polyclonal to LRPPRC/GP130 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human LRPPRC/GP130 aa 611-907.
Database link: P42704 -
Positive control
- WB: H1299, HeLa whole cell lysate. IHC-P: SW480 Xenograft, human colon cancer tissue. ICC/IF: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 158 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/10000. Predicted molecular weight: 158 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA. -
Tissue specificity
Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes. -
Involvement in disease
Leigh syndrome French-Canadian type -
Sequence similarities
Contains 20 PPR (pentatricopeptide) repeats. -
Cellular localization
Mitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial. - Information by UniProt
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Database links
- Entrez Gene: 10128 Human
- Entrez Gene: 313867 Rat
- Omim: 607544 Human
- SwissProt: P42704 Human
- SwissProt: Q5SGE0 Rat
- Unigene: 368084 Human
- Unigene: 92051 Rat
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Alternative names
- 130 kDa leucine-rich protein antibody
- gp130 antibody
- Leucine-rich PPR motif-containing protein antibody
see all
Images
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All lanes : Anti-LRPPRC/GP130 antibody (ab97505) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 158 kDa
5% SDS-PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa using ab97505 at 1/200 dilution. Lower panel is also merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded SW480 Xenograft using ab97505 at 1/100 dilution.
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Paraffin embedded human colon cancer tissue stained for LRP130 using ab97505 at 1/100 at dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab97505 has been referenced in 13 publications.
- Ke J et al. [Comprehensive Analysis of the Relationship between m6A Methylation Patterns and Immune Microenvironment in Lung Adenocarcinoma]. Zhongguo Fei Ai Za Zhi 25:311-322 (2022). PubMed: 35599007
- Hu Y et al. LRPPRC contributes to the cisplatin resistance of lung cancer cells by regulating MDR1 expression. Oncol Rep 45:N/A (2021). PubMed: 33649818
- Saleem I et al. The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export. Mol Cell Biol 41:e0010321 (2021). PubMed: 33941617
- Wurlitzer M et al. Mass Spectrometric Comparison of HPV-Positive and HPV-Negative Oropharyngeal Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32545200
- Islam H et al. Fiber-specific and whole-muscle LRP130 expression in rested, exercised, and fasted human skeletal muscle. Pflugers Arch 472:375-384 (2020). PubMed: 32065259