Overview

  • Product name
  • Description
    Rabbit polyclonal to LRRC47
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen, corresponding to amino acids 348-469 of Human LRRC47 (Q8N1G4).

  • Positive control
    • Human colon tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-LRRC47 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121283 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical staining of formalin fixed and paraffin embedded Human colon tissue labelled with ab121283 at 1/100 dilution.
  • All lanes : Anti-LRRC47 antibody (ab121283) at 1/250 dilution

    Lane 1 : RT-4
    Lane 2 : U-251 MG
    Lane 3 : Human plasma
    Lane 4 : Liver
    Lane 5 : Tonsil

References for Anti-LRRC47 antibody (ab121283)

ab121283 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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