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Rabbit polyclonal to LRRC47
Mouse, Rat, Human
antigen, corresponding to amino acids 348-469 of Human LRRC47 (Q8N1G4).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Contains 7 LRR (leucine-rich) repeats.
Information by UniProt
- 2900010D03Rik antibody
- AA960559 antibody
- Leucine-rich repeat-containing protein 47 antibody
Immunocytochemistry/ Immunofluorescence - Anti-LRRC47 antibody (ab121283)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121283 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC47 antibody (ab121283)
Immunohistochemical staining of formalin fixed and paraffin embedded Human colon tissue labelled with ab121283 at 1/100 dilution.
Western blot - Anti-LRRC47 antibody (ab121283)
All lanes : Anti-LRRC47 antibody (ab121283) at 1/250 dilution
Lane 1 : RT-4
Lane 2 : U-251 MG
Lane 3 : Human plasma
Lane 4 : Liver
Lane 5 : Tonsil
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"