Overview

  • Product nameAnti-LRRC50 antibody
    See all LRRC50 primary antibodies
  • Description
    Rabbit polyclonal to LRRC50
  • Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A synthetic peptide corresponding to a region of Human LRRC50. The immunogen is found within the following sequence which corresponds to aa 151-200 of the human protein: TELRCLFLQMNLLRKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTL .

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:1562500.

ICC/IF Use at an assay dependent concentration. PubMed: 23599692
IHC-P Use at an assay dependent concentration. PubMed: 23599692

Target

  • RelevanceLRRC50 contains six LRR (leucine-rich) repeats. It is proposed that LRRC50 is a novel candidate gene for human cystic kidney disease, involved in regulation of microtubule-based cilia and actin-based brush border microvilli.
  • Cellular localizationCell projection; cilium. Cytoplasm. Cytoplasm; cytoskeleton, spindle pole. Note: In HEK293T cells, it is diffusely cytoplasmic and concentrates at the mitotic spindle poles, while in MDCK cells, it localizes in the cilium. In vivo, this protein is probably restricted to the cilium.
  • Database links
  • Alternative names
    • CILD13 antibody
    • DNAAF1 antibody
    • Dynein assembly factor 1, axonemal antibody
    • Leucine rich repeat containing 50 antibody
    • ODA7 antibody
    see all

Anti-LRRC50 antibody images

  • Anti-LRRC50 antibody (ab75163) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 80 kDa

References for Anti-LRRC50 antibody (ab75163)

This product has been referenced in:
  • Basten SG  et al. Mutations in LRRC50 predispose zebrafish and humans to seminomas. PLoS Genet 9:e1003384 (2013). IHC-P, ICC/IF ; Human, Zebrafish . Read more (PubMed: 23599692) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"