Overview

  • Product nameAnti-LRRFIP1 antibody
    See all LRRFIP1 primary antibodies
  • Description
    Mouse monoclonal to LRRFIP1
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 675-784 of human LRRFIP1 (NP_004726).

  • Positive control
    • HeLa nuclear cell lysate; recombinant fragment of human LRRFIP1; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab77598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged LRRFIP1 is approximately 0.03 ng/ml when used as a capture antibody.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionTranscriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the LRRFIP family.
  • Developmental stageWidely expressed in fetal tissues.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLAP 1 antibody
    • FLAP1 antibody
    • FLIIAP 1 antibody
    • FLIIAP1 antibody
    • GC binding factor 2 antibody
    • GC-binding factor 2 antibody
    • GCF 2 antibody
    • GCF2 antibody
    • HUFI 1 antibody
    • HUFI1 antibody
    • Leucine rich repeat (in FLII) interacting protein 1 antibody
    • Leucine rich repeat flightless interacting protein 1 antibody
    • Leucine-rich repeat flightless-interacting protein 1 antibody
    • LRR FLII interacting protein 1 antibody
    • LRR FLII-interacting protein 1 antibody
    • LRRF1_HUMAN antibody
    • LRRFIP 1 antibody
    • Lrrfip1 antibody
    • MGC10947 antibody
    • MGC119738 antibody
    • MGC119739 antibody
    • NEDD8 conjugating enzyme antibody
    • TAR RNA interacting protein antibody
    • TAR RNA-interacting protein antibody
    • Transcription factor 9 like antibody
    • TRIP antibody
    see all

Anti-LRRFIP1 antibody images

  • Anti-LRRFIP1 antibody (ab77598) at 1/500 dilution + recombinant fragment of human LRRFIP1 (the immunogen) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 80 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-LRRFIP1 antibody (ab77598) at 1/500 dilution + HeLa nuclear extract at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 80 kDa
    Observed band size : 80,150 kDa (why is the actual band size different from the predicted?)
  • ab77598, at 10 µg/ml, staining LRRFIP1 in HeLa cells by Immunofluorescence.
  • IHC image of ab77598 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77598, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Overlay histogram showing HeLa cells stained with ab75598 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75598, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-LRRFIP1 antibody (ab77598)

ab77598 has not yet been referenced specifically in any publications.

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