Overview

  • Product nameAnti-LRRN3 antibody
    See all LRRN3 primary antibodies
  • Description
    Rabbit polyclonal to LRRN3
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (ELYINHNLLS TISPGAFIGL HNLLRLHLNS NRLQMINSKW FDALPNLEIL) of Human LRRN3 (NP_001093130).

  • Positive control
    • Placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.

Target

Anti-LRRN3 antibody images

  • Anti-LRRN3 antibody (ab82657) at 1 µg/ml (5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • ab82657 staining LRRN3 on E14.5 mouse embryo (intercostal region) by IHC-Fr (left). The tissue was formaldehyde fixed and then blocked for 1 hour. The primary antibody was diluted 1/300 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary. Nuclei were stained with Hoechst 33342 (right).

    See Abreview

References for Anti-LRRN3 antibody (ab82657)

ab82657 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E14.5 wildtype intercostal region)
Specification E14.5 wildtype intercostal region
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
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Submitted Jul 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"