The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. Regulates surface expression of AMPA receptors. Acts as a ligand for the presynaptic receptors NRXN1-A and NRXN1-B.
Expressed in neuronal tissues.
Belongs to the LRRTM family. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.
Synaptogenic effects are mediated by the extracellular LRR region.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labelling LRRTM2 with ab133208 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.