Overview

  • Product name
    Anti-LRTM1 antibody
  • Description
    Rabbit polyclonal to LRTM1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 139-286 of Human LRTM1.

  • Positive control
    • Human cerebellum tissue. HEK293T cells ovrexpressing LRTM1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Sequence similarities
    Contains 5 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HT017 antibody
    • Leucine rich repeats and transmembrane domains 1 antibody
    • Leucine-rich repeat and transmembrane domain-containing protein 1 antibody
    • Lrtm1 antibody
    • LRTM1_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121409 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121409 at 1/20 dilution staining LRTM1 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • All lanes : Anti-LRTM1 antibody (ab121409) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : HEK293T cells overexpressing LRTM1 (Co-expressed with a C-terminal myc-DDK tag) in mammalian HEK293T cells

    Developed using the ECL technique

References

ab121409 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121409.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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