Overview

  • Product name
  • Description
    Rabbit polyclonal to LRWD1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LRWD1.

  • Positive control
    • This antibody gave a positive signal in Human testis tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).

Target

  • Function
    Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
  • Sequence similarities
    Belongs to the LRWD1 family.
    Contains 3 LRR (leucine-rich) repeats.
    Contains 5 WD repeats.
  • Domain
    The WD repeats are required for the interaction with ORC components.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200011O22Rik antibody
    • AU042569 antibody
    • AW548074 antibody
    • DKFZp434K1815 antibody
    • Leucine rich repeats and WD repeat domain containing 1 antibody
    • Leucine-rich repeat and WD repeat-containing protein 1 antibody
    • LRWD1 antibody
    • LRWD1_HUMAN antibody
    • ORC-associated protein antibody
    • ORCA antibody
    • Origin recognition complex-associated protein antibody
    see all

Anti-LRWD1 antibody images

  • Anti-LRWD1 antibody (ab107779) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Additional bands at : 190 kDa,235 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-LRWD1 antibody (ab107779)

ab107779 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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